00:00:00Introduction
00:00:55Background on Iterative Mapping and tau
00:05:08Takeaway one: the information is in the intact molecule
00:07:43Takeaway two: remodeling, not simply more phosphorylation
00:09:24Takeaway three: biological context matters
00:10:55Takeaway four: Single-molecule analysis reveals biology hidden from bulk measurement and is ready for primetime
00:13:07Takeaway five: Co-occurring modification is extensive
00:14:05Takeaway six – There is order and timing to combinatorial modification
00:15:57Iterative Mapping accessibility and un-matched quantitative capabilities
00:17:33The pseudo-Western: one site at a time versus the full proteoform view
00:19:19Applications: Adapting Iterative Mapping to proteoforms beyond tau
00:19:56Applications: Telling tauopathies apart
00:21:38Applications: Investigating selective vulnerability to neurodegeneration
00:23:12Applications: Investigating brain injury
00:23:52Applications: Investigating the potential of human-derived model systems such as brain organoids
00:25:48Applications: Guiding drug development
00:26:50Summary: A foundational technology with limitless applications
00:30:51Outro
